0 mcherry cassette (Addgene inc)
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0 mcherry cassette
0 Mcherry Cassette, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/maid+mcherry+cassette/pMK293+(mAID-mCherry2-Hygro)+(Plasmid+%2372831)/bio_rxiv__64898__2026__02__17__706355-134-0-3
Average 93 stars, based on 18 article reviews
0 Mcherry Cassette, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/maid+mcherry+cassette/pMK293+(mAID-mCherry2-Hygro)+(Plasmid+%2372831)/bio_rxiv__64898__2026__02__17__706355-134-0-3
Average 93 stars, based on 18 article reviews
0 mcherry cassette - by Bioz Stars,
2026-11
93/100 stars
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Plasmid Preparation:Article Title: A novel auxin-inducible degron system for rapid, cell cycle-specific targeted proteolysis Article Snippet: To construct pAAVS1-ROLECCS AS, pAAVS1-ROLECCS G1, and pAAVS1-ROLECCS G2 plasmids, multiple fragments were PCR ampli ed from different donor plasmids and assembled as follow: pMK232 CMV-OsTIR1-PURO (Addgene #7283437) was used as donor plasmid for the expression of OsTIR1 from the AAVS1 locus, the mEmerald tag was PCR ampli ed from mEmerald-PLK1-N-16 vector (Addgene #54234; http://n2t.net/addgene:54234 ; RRID:Addgene_54234), while pEN435 - pCAGGS-TagBFP-hGeminin-2AmCherry-hCdt1-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt Tigre targeting (Addgene #9213928) was used as template for both hGeminin and hCdt1 tags. .. The vector for the mAID-mCherry expression was generated Page 14/30 using the pEGFP-C1 backbone (Clontech), replacing the GFP gene with the Article Title: Nucleolin acute degradation reveals novel functions in cell cycle progression and division in TNBC Article Snippet: To generate the donor plasmids for endogenous NCL editing, the genomic region (∼2000 bp) surrounding the natural start codon on exon 1 was cloned into the pUC19 vector (New England Biolabs, N3041S) by Gibson Assembly. .. The Article Title: A novel auxin-inducible degron system for rapid, cell cycle-specific targeted proteolysis Article Snippet: To construct pAAVS1-ROLECCS AS, pAAVS1-ROLECCS G1, and pAAVS1-ROLECCS G2 plasmids, multiple fragments were PCR ampli ed from different donor plasmids and assembled as follow: pMK232 CMV-OsTIR1-PURO (Addgene #7283437) was used as donor plasmid for the expression of OsTIR1 from the AAVS1 locus, the mEmerald tag was PCR ampli ed from mEmerald-PLK1-N-16 vector (Addgene #54234; http://n2t.net/addgene:54234 ; RRID:Addgene_54234), while pEN435 - pCAGGS-TagBFP-hGeminin-2AmCherry-hCdt1-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt Tigre targeting (Addgene #9213928) was used as template for both hGeminin and hCdt1 tags. .. The vector for the mAID-mCherry expression was generated Page 14/30 using the pEGFP-C1 backbone (Clontech), replacing the GFP gene with the Article Title: A novel auxin-inducible degron system for rapid, cell cycle-specific targeted proteolysis Article Snippet: To construct pAAVS1-ROLECCS AS, pAAVS1-ROLECCS G1, and pAAVS1-ROLECCS G2 plasmids, multiple fragments were PCR amplified from different donor plasmids and assembled as follow: pMK232 CMV-OsTIR1-PURO (Addgene #72834 ) was used as donor plasmid for the expression of OsTIR1 from the AAVS1 locus, the mEmerald tag was PCR amplified from mEmerald-PLK1-N-16 vector (Addgene #54234; http://n2t.net/addgene:54234 ; RRID:Addgene_54234), while pEN435 - pCAGGS-TagBFP-hGeminin-2A-mCherry-hCdt1- rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt Tigre targeting (Addgene #92139 ) was used as template for both hGeminin and hCdt1 tags. .. The vector for the mAID-mCherry expression was generated using the pEGFP-C1 backbone (Clontech), replacing the GFP gene with the Article Title: A novel auxin-inducible degron system for rapid, cell cycle-specific targeted proteolysis. Article Snippet: To construct pAAVS1-ROLECCS AS, pAAVS1ROLECCS G1, and pAAVS1-ROLECCS G2 plasmids, multiple fragments were PCR amplified from different donor plasmids and assembled as follow: pMK232 CMV-OsTIR1-PURO (Addgene #72834 [37]) was used as donor plasmid for the expression of OsTIR1 from the AAVS1 locus, themEmerald tag was PCR amplified from mEmerald-PLK1-N-16 vector (Addgene #54234; http://n2t.net/addgene:54234; RRID:Addgene_54234), while pEN435 - pCAGGS-TagBFP-hGeminin-2A-mCherry-hCdt1-rbgpA-Frt-PGK-EM7-PuroRbpA-Frt Tigre targeting (Addgene #92139 [28]) was used as template for both hGeminin and hCdt1 tags. .. The vector for the mAID-mCherry expression was generated using the pEGFP-C1 backbone (Clontech), replacing the GFP gene with the Expressing:Article Title: A novel auxin-inducible degron system for rapid, cell cycle-specific targeted proteolysis Article Snippet: To construct pAAVS1-ROLECCS AS, pAAVS1-ROLECCS G1, and pAAVS1-ROLECCS G2 plasmids, multiple fragments were PCR ampli ed from different donor plasmids and assembled as follow: pMK232 CMV-OsTIR1-PURO (Addgene #7283437) was used as donor plasmid for the expression of OsTIR1 from the AAVS1 locus, the mEmerald tag was PCR ampli ed from mEmerald-PLK1-N-16 vector (Addgene #54234; http://n2t.net/addgene:54234 ; RRID:Addgene_54234), while pEN435 - pCAGGS-TagBFP-hGeminin-2AmCherry-hCdt1-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt Tigre targeting (Addgene #9213928) was used as template for both hGeminin and hCdt1 tags. .. The vector for the mAID-mCherry expression was generated Page 14/30 using the pEGFP-C1 backbone (Clontech), replacing the GFP gene with the Article Title: A novel auxin-inducible degron system for rapid, cell cycle-specific targeted proteolysis Article Snippet: To construct pAAVS1-ROLECCS AS, pAAVS1-ROLECCS G1, and pAAVS1-ROLECCS G2 plasmids, multiple fragments were PCR ampli ed from different donor plasmids and assembled as follow: pMK232 CMV-OsTIR1-PURO (Addgene #7283437) was used as donor plasmid for the expression of OsTIR1 from the AAVS1 locus, the mEmerald tag was PCR ampli ed from mEmerald-PLK1-N-16 vector (Addgene #54234; http://n2t.net/addgene:54234 ; RRID:Addgene_54234), while pEN435 - pCAGGS-TagBFP-hGeminin-2AmCherry-hCdt1-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt Tigre targeting (Addgene #9213928) was used as template for both hGeminin and hCdt1 tags. .. The vector for the mAID-mCherry expression was generated Page 14/30 using the pEGFP-C1 backbone (Clontech), replacing the GFP gene with the Article Title: A novel auxin-inducible degron system for rapid, cell cycle-specific targeted proteolysis Article Snippet: To construct pAAVS1-ROLECCS AS, pAAVS1-ROLECCS G1, and pAAVS1-ROLECCS G2 plasmids, multiple fragments were PCR amplified from different donor plasmids and assembled as follow: pMK232 CMV-OsTIR1-PURO (Addgene #72834 ) was used as donor plasmid for the expression of OsTIR1 from the AAVS1 locus, the mEmerald tag was PCR amplified from mEmerald-PLK1-N-16 vector (Addgene #54234; http://n2t.net/addgene:54234 ; RRID:Addgene_54234), while pEN435 - pCAGGS-TagBFP-hGeminin-2A-mCherry-hCdt1- rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt Tigre targeting (Addgene #92139 ) was used as template for both hGeminin and hCdt1 tags. .. The vector for the mAID-mCherry expression was generated using the pEGFP-C1 backbone (Clontech), replacing the GFP gene with the Article Title: A novel auxin-inducible degron system for rapid, cell cycle-specific targeted proteolysis. Article Snippet: To construct pAAVS1-ROLECCS AS, pAAVS1ROLECCS G1, and pAAVS1-ROLECCS G2 plasmids, multiple fragments were PCR amplified from different donor plasmids and assembled as follow: pMK232 CMV-OsTIR1-PURO (Addgene #72834 [37]) was used as donor plasmid for the expression of OsTIR1 from the AAVS1 locus, themEmerald tag was PCR amplified from mEmerald-PLK1-N-16 vector (Addgene #54234; http://n2t.net/addgene:54234; RRID:Addgene_54234), while pEN435 - pCAGGS-TagBFP-hGeminin-2A-mCherry-hCdt1-rbgpA-Frt-PGK-EM7-PuroRbpA-Frt Tigre targeting (Addgene #92139 [28]) was used as template for both hGeminin and hCdt1 tags. .. The vector for the mAID-mCherry expression was generated using the pEGFP-C1 backbone (Clontech), replacing the GFP gene with the Generated:Article Title: A novel auxin-inducible degron system for rapid, cell cycle-specific targeted proteolysis Article Snippet: To construct pAAVS1-ROLECCS AS, pAAVS1-ROLECCS G1, and pAAVS1-ROLECCS G2 plasmids, multiple fragments were PCR ampli ed from different donor plasmids and assembled as follow: pMK232 CMV-OsTIR1-PURO (Addgene #7283437) was used as donor plasmid for the expression of OsTIR1 from the AAVS1 locus, the mEmerald tag was PCR ampli ed from mEmerald-PLK1-N-16 vector (Addgene #54234; http://n2t.net/addgene:54234 ; RRID:Addgene_54234), while pEN435 - pCAGGS-TagBFP-hGeminin-2AmCherry-hCdt1-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt Tigre targeting (Addgene #9213928) was used as template for both hGeminin and hCdt1 tags. .. The vector for the mAID-mCherry expression was generated Page 14/30 using the pEGFP-C1 backbone (Clontech), replacing the GFP gene with the Article Title: A novel auxin-inducible degron system for rapid, cell cycle-specific targeted proteolysis Article Snippet: To construct pAAVS1-ROLECCS AS, pAAVS1-ROLECCS G1, and pAAVS1-ROLECCS G2 plasmids, multiple fragments were PCR ampli ed from different donor plasmids and assembled as follow: pMK232 CMV-OsTIR1-PURO (Addgene #7283437) was used as donor plasmid for the expression of OsTIR1 from the AAVS1 locus, the mEmerald tag was PCR ampli ed from mEmerald-PLK1-N-16 vector (Addgene #54234; http://n2t.net/addgene:54234 ; RRID:Addgene_54234), while pEN435 - pCAGGS-TagBFP-hGeminin-2AmCherry-hCdt1-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt Tigre targeting (Addgene #9213928) was used as template for both hGeminin and hCdt1 tags. .. The vector for the mAID-mCherry expression was generated Page 14/30 using the pEGFP-C1 backbone (Clontech), replacing the GFP gene with the Article Title: A novel auxin-inducible degron system for rapid, cell cycle-specific targeted proteolysis Article Snippet: To construct pAAVS1-ROLECCS AS, pAAVS1-ROLECCS G1, and pAAVS1-ROLECCS G2 plasmids, multiple fragments were PCR amplified from different donor plasmids and assembled as follow: pMK232 CMV-OsTIR1-PURO (Addgene #72834 ) was used as donor plasmid for the expression of OsTIR1 from the AAVS1 locus, the mEmerald tag was PCR amplified from mEmerald-PLK1-N-16 vector (Addgene #54234; http://n2t.net/addgene:54234 ; RRID:Addgene_54234), while pEN435 - pCAGGS-TagBFP-hGeminin-2A-mCherry-hCdt1- rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt Tigre targeting (Addgene #92139 ) was used as template for both hGeminin and hCdt1 tags. .. The vector for the mAID-mCherry expression was generated using the pEGFP-C1 backbone (Clontech), replacing the GFP gene with the Article Title: A novel auxin-inducible degron system for rapid, cell cycle-specific targeted proteolysis. Article Snippet: To construct pAAVS1-ROLECCS AS, pAAVS1ROLECCS G1, and pAAVS1-ROLECCS G2 plasmids, multiple fragments were PCR amplified from different donor plasmids and assembled as follow: pMK232 CMV-OsTIR1-PURO (Addgene #72834 [37]) was used as donor plasmid for the expression of OsTIR1 from the AAVS1 locus, themEmerald tag was PCR amplified from mEmerald-PLK1-N-16 vector (Addgene #54234; http://n2t.net/addgene:54234; RRID:Addgene_54234), while pEN435 - pCAGGS-TagBFP-hGeminin-2A-mCherry-hCdt1-rbgpA-Frt-PGK-EM7-PuroRbpA-Frt Tigre targeting (Addgene #92139 [28]) was used as template for both hGeminin and hCdt1 tags. .. The vector for the mAID-mCherry expression was generated using the pEGFP-C1 backbone (Clontech), replacing the GFP gene with the Derivative Assay:Article Title: A novel auxin-inducible degron system for rapid, cell cycle-specific targeted proteolysis Article Snippet: To construct pAAVS1-ROLECCS AS, pAAVS1-ROLECCS G1, and pAAVS1-ROLECCS G2 plasmids, multiple fragments were PCR ampli ed from different donor plasmids and assembled as follow: pMK232 CMV-OsTIR1-PURO (Addgene #7283437) was used as donor plasmid for the expression of OsTIR1 from the AAVS1 locus, the mEmerald tag was PCR ampli ed from mEmerald-PLK1-N-16 vector (Addgene #54234; http://n2t.net/addgene:54234 ; RRID:Addgene_54234), while pEN435 - pCAGGS-TagBFP-hGeminin-2AmCherry-hCdt1-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt Tigre targeting (Addgene #9213928) was used as template for both hGeminin and hCdt1 tags. .. The vector for the mAID-mCherry expression was generated Page 14/30 using the pEGFP-C1 backbone (Clontech), replacing the GFP gene with the Article Title: A novel auxin-inducible degron system for rapid, cell cycle-specific targeted proteolysis Article Snippet: To construct pAAVS1-ROLECCS AS, pAAVS1-ROLECCS G1, and pAAVS1-ROLECCS G2 plasmids, multiple fragments were PCR ampli ed from different donor plasmids and assembled as follow: pMK232 CMV-OsTIR1-PURO (Addgene #7283437) was used as donor plasmid for the expression of OsTIR1 from the AAVS1 locus, the mEmerald tag was PCR ampli ed from mEmerald-PLK1-N-16 vector (Addgene #54234; http://n2t.net/addgene:54234 ; RRID:Addgene_54234), while pEN435 - pCAGGS-TagBFP-hGeminin-2AmCherry-hCdt1-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt Tigre targeting (Addgene #9213928) was used as template for both hGeminin and hCdt1 tags. .. The vector for the mAID-mCherry expression was generated Page 14/30 using the pEGFP-C1 backbone (Clontech), replacing the GFP gene with the Article Title: A novel auxin-inducible degron system for rapid, cell cycle-specific targeted proteolysis Article Snippet: To construct pAAVS1-ROLECCS AS, pAAVS1-ROLECCS G1, and pAAVS1-ROLECCS G2 plasmids, multiple fragments were PCR amplified from different donor plasmids and assembled as follow: pMK232 CMV-OsTIR1-PURO (Addgene #72834 ) was used as donor plasmid for the expression of OsTIR1 from the AAVS1 locus, the mEmerald tag was PCR amplified from mEmerald-PLK1-N-16 vector (Addgene #54234; http://n2t.net/addgene:54234 ; RRID:Addgene_54234), while pEN435 - pCAGGS-TagBFP-hGeminin-2A-mCherry-hCdt1- rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt Tigre targeting (Addgene #92139 ) was used as template for both hGeminin and hCdt1 tags. .. The vector for the mAID-mCherry expression was generated using the pEGFP-C1 backbone (Clontech), replacing the GFP gene with the Article Title: A novel auxin-inducible degron system for rapid, cell cycle-specific targeted proteolysis. Article Snippet: To construct pAAVS1-ROLECCS AS, pAAVS1ROLECCS G1, and pAAVS1-ROLECCS G2 plasmids, multiple fragments were PCR amplified from different donor plasmids and assembled as follow: pMK232 CMV-OsTIR1-PURO (Addgene #72834 [37]) was used as donor plasmid for the expression of OsTIR1 from the AAVS1 locus, themEmerald tag was PCR amplified from mEmerald-PLK1-N-16 vector (Addgene #54234; http://n2t.net/addgene:54234; RRID:Addgene_54234), while pEN435 - pCAGGS-TagBFP-hGeminin-2A-mCherry-hCdt1-rbgpA-Frt-PGK-EM7-PuroRbpA-Frt Tigre targeting (Addgene #92139 [28]) was used as template for both hGeminin and hCdt1 tags. .. The vector for the mAID-mCherry expression was generated using the pEGFP-C1 backbone (Clontech), replacing the GFP gene with the Selection:Article Title: A novel auxin-inducible degron system for rapid, cell cycle-specific targeted proteolysis Article Snippet: Secondly, the homology arms containing plasmid was mutated using QuikChange II XL Site-Directed Mutagenesis Kit (Agilent, #200522) to delete the single-guide RNA (sgRNA) recognition sequence, to prevent Cas9 from re-cutting after homology-directed repair-mediated insertion at the desired genetic locus. .. Finally the Article Title: A novel auxin-inducible degron system for rapid, cell cycle-specific targeted proteolysis. Article Snippet: Secondly, the homology arms containing plasmid was mutated using QuickChange II XL Site-Directed Mutagenesis Kit (Agilent, #200522) to delete the single-guide RNA (sgRNA) recognition sequence, to prevent Cas9 from re-cutting after homology-directed repairmediated insertion at the desired genetic locus. .. Finally the Marker:Article Title: A novel auxin-inducible degron system for rapid, cell cycle-specific targeted proteolysis Article Snippet: Secondly, the homology arms containing plasmid was mutated using QuikChange II XL Site-Directed Mutagenesis Kit (Agilent, #200522) to delete the single-guide RNA (sgRNA) recognition sequence, to prevent Cas9 from re-cutting after homology-directed repair-mediated insertion at the desired genetic locus. .. Finally the Article Title: A novel auxin-inducible degron system for rapid, cell cycle-specific targeted proteolysis. Article Snippet: Secondly, the homology arms containing plasmid was mutated using QuickChange II XL Site-Directed Mutagenesis Kit (Agilent, #200522) to delete the single-guide RNA (sgRNA) recognition sequence, to prevent Cas9 from re-cutting after homology-directed repairmediated insertion at the desired genetic locus. .. Finally the Amplification:Article Title: A novel auxin-inducible degron system for rapid, cell cycle-specific targeted proteolysis Article Snippet: Secondly, the homology arms containing plasmid was mutated using QuikChange II XL Site-Directed Mutagenesis Kit (Agilent, #200522) to delete the single-guide RNA (sgRNA) recognition sequence, to prevent Cas9 from re-cutting after homology-directed repair-mediated insertion at the desired genetic locus. .. Finally the Article Title: Nucleolin acute degradation reveals novel functions in cell cycle progression and division in TNBC Article Snippet: To generate the donor plasmids for endogenous NCL editing, the genomic region (∼2000 bp) surrounding the natural start codon on exon 1 was cloned into the pUC19 vector (New England Biolabs, N3041S) by Gibson Assembly. .. The Article Title: A novel auxin-inducible degron system for rapid, cell cycle-specific targeted proteolysis. Article Snippet: Secondly, the homology arms containing plasmid was mutated using QuickChange II XL Site-Directed Mutagenesis Kit (Agilent, #200522) to delete the single-guide RNA (sgRNA) recognition sequence, to prevent Cas9 from re-cutting after homology-directed repairmediated insertion at the desired genetic locus. .. Finally the Clone Assay:Article Title: A novel auxin-inducible degron system for rapid, cell cycle-specific targeted proteolysis Article Snippet: Secondly, the homology arms containing plasmid was mutated using QuikChange II XL Site-Directed Mutagenesis Kit (Agilent, #200522) to delete the single-guide RNA (sgRNA) recognition sequence, to prevent Cas9 from re-cutting after homology-directed repair-mediated insertion at the desired genetic locus. .. Finally the Article Title: A novel auxin-inducible degron system for rapid, cell cycle-specific targeted proteolysis. Article Snippet: Secondly, the homology arms containing plasmid was mutated using QuickChange II XL Site-Directed Mutagenesis Kit (Agilent, #200522) to delete the single-guide RNA (sgRNA) recognition sequence, to prevent Cas9 from re-cutting after homology-directed repairmediated insertion at the desired genetic locus. .. Finally the |